---
identifier: Wm82.gnm6.ann1.expr.Magellan.Pelaez-Vico_Sinha_2023 

provenance: "The files in this directory derive from supplementary data from Pelaez-Vico et al., Integrative phenotypic-transcriptomic analysis of soybean plants subjected to multifactorialstress combination. bioRxiv. The files here are held as part of the SoyBase, LegumeInfo, and LegumeFederation projects, and are made available here for the purpose of reproducibility of analyses at these sites (e.g. gene family alignments and phylogenies, genome browsers, etc.) and for further use by researchers, as that research extends other analyses at the SoyBase, LegumeInfo, and Legume Federation project(s). If you are conducting research on large-scale data sets for this species, please consider retrieving the data from the primary repositories. If you use the data in the present directory, please 1) please cite the data appropriately - generally referring to the original publications for this data; and if you make use of any significant modifications in the files (noted below under Transformations where applicable), then please also cite the respective database project(s) related to this directory."

synopsis: "Combined phenotypic and transcriptomic analysis of soybean plants subjected to multifactorial stress combination (Pelaez-Vico et al.)"

scientific_name: Glycine max  
  
taxid: 3847

scientific_name_abbrev: glyma

genotype:
  - Magellan

expression_unit: TPM

description: "Soybean plants were subjected to a multifactorial stress combination of up to five different stresses (water deficit, salinity, low phosphate, acidity, and cadmium), in an increasing level of complexity. All stresses were applied at the beginning of the experiment except for water deficit stress that was imposed after 21 days. Leaves and flowers were collected from 5-7 different plants under the mentioned stress conditions and after 10 days of starting water deficit conditions. Differential gene expression compared to control was studied using RNAseq method for all the possible stress combinations. Overall design: Tissues from soybean plants (31 days old) subjected to water deficit, salinity, low phosphate, acidity, and cadmium alone or in combination of 2, 3, 4 and 5 stresses were collected in liquid nitrogen. Total RNA was isolated and mRNA profile was generated by deep sequencing using NovaSeq 6000 PE150 (Pelaez-Vico et al.). "

original_file_creation_date: "2024-05-29"

local_file_creation_date: "2024-05-29"

dataset_release_date: "2023-07-19"

publication_doi: 10.1101/2023.08.15.553447

publication_title: "An integrated transcriptome atlas of the crop model Glycine max, and its use in comparative analyses in plants."

bioproject: PRJNA996630

geoseries: GSE237798

data_curators: Andrew Farmer, Evan Lavelle

public_access_level: public

license: open

keywords: soybean, stressors, salinity, phosphate, acidity, cadmium

citation: "Pelaez-Vico et al., 2023, Integrative phenotypic-transcriptomic analysis of soybean plants subjected to multifactorialstress combination. bioRxiv."

contributors: "Maria Angeles Pelaez-Vico, Ranjita Sinha, Sai Preethi INduri, Zhen Lyu, Sai Darahas Venigalla, Dinesh Vasireddy, Pallav Singh, Manish Sridhar Immadi, Lidia S. Pascual, Benjamin Shostak, David Mendoza-Cozatl, Trupti Joshi, Felix B. Fritschi, Sara I. Zandalinas, Ron Mittler."