## DOCUMENT 1 ##
---
scientific_name: Glycine soja
gene_symbols:
  - GsERD15B
gene_symbol_long: Early Responsive to Dehydration 15B
gene_model_pub_name: XP_028191281.1
gene_model_full_id: glyso.PI483463.gnm1.ann1.GlysoPI483463.11G164100
confidence: 4
curators:
  - Greg Murrell
  - Steven Cannon
  - Scott Kalberer
comments:
  - A seven-base pair deletion in the GsERD15B promoter was discovered in the majority (42) of the 48 wild Glycine soja accessions with enhanced salt tolerance.
  - Increased expression of GsERD15B in response to salt stress was associated with up-regulation of two GmPAB genes (GmPAB-14G and GmPAB-17G) and known stress-related genes including ABA-related genes (GmABI1, GmABI2, GmbZIP1), a proline synthesis-related gene (GmP5CS), a catalase peroxidase gene (GmCAT4), water loss-related genes (GmPIP1:6, GmMYB84), and an ion exchanger encoding gene (GmSOS1).
  - GsERD15B overexpression enhanced the promoter activities of GmABI2, GmP5CS and GmbZIP1 in tobacco leaf assays.
  - GsERD15B encodes an ERD15 protein with transcriptional activation function.  The ERD15 contains a conserved PAM2 domain in the N-terminus that mediates its interaction with the PABC domain of poly(A)-binding (PAB) proteins.
phenotype_summary: Gene overexpression in Glycine enhanced salt tolerance probably by increasing the expression levels of genes related to ABA-signalling, proline content, catalase peroxidase, dehydration response and cation transport.
traits:
  - entity_name: salt tolerance
    entity: TO:0006001
  - relation_name: positively regulates
    relation: RO:0002213
references:
  - citation: Jin, Sun et al., 2021
    doi: 10.1111/pbi.13536
    pmid: 33368860


## DOCUMENT 2 ##
---
scientific_name: Glycine soja
gene_symbols:
  - GmCHX1
gene_symbol_long: cation/H(+) antiporter 1
gene_model_pub_name: Glysoja01g005509
gene_model_full_id: glyso.PI483463.gnm1.ann1.GlysoPI483463.03G135400
confidence: 4
comments:
  - Gene Glysoja01g005509 is newly sequenced from Glycine soja W05 and is not in the annotation glyso.W05.gnm1.ann1.
  - The newly named gene Glysoja01g005509 has a different format than others in that annotation, which have a dot, e.g. Glysoja.01G001001
  - The gene GmCHX1 is associated with salt tolerance in Glycine soja W05
  - The gene glyso.PI483463.gnm1.ann1.GlysoPI483463.03G135400 has a full-length 100% match with the reported Glysoja01g005509
  - The reported sequence is >KF879911.1 Glycine soja CHX1 mRNA, complete cds ATGACGTTCAACGCGAGCACCATCACAACGGCGTCGGAAGGAGCCTGGCAGGGCGATAATCCCCTGAACC ACGCTCTTCCTTTGTTGATCGTTCAAACCATCCTCGTAGTCTTCGTGAGCCGCACACTCGCCTTTCTCCT CAAACCCTTTCGTCAACCTAAAGTTGTCGCCGAGATTATTGGTGGAATTTTGTTGGGGCCTTCTGCTATT GGGCGCAACAAGAAATTCATGCACATAGTGTTCCCAGCATGGAGCACTACCATGCTGGAATCAGTGGCAA GCTTCGGCCTCTTATTCTATCTATTTCTGGTGGGCCTAGAGCTCGACTTTCGCACCATTCGCCGGAGCGG CAAGCAAGCCTTCAACATCGCGGTGGCCGGAATAACCCTCCCCTTCATCTGCGCCGTGGGAGTAACGTTC CTTCTCCAGAGAGCCATCCGCTCTGAAAACCATAACATAGGGTACGTTCAGCACTTCGTGTTCTTAGGGG TATCTCTGTCCATCACGGCTTTCCCTGTGCTCGCGCGCATCTTAGCGGAGCTCAAACTGCTGACCACACG TGTGGGAGAAACCGCCATGGCGGCTGCAGCCTTCAACGACGTCGCTGCGTGGGTTTTGTTGGCCTTGGCG GTGGCTTTGGCTGGCCAGGGACACAAAAGCAGCTTGTTGACATCAATATGGGTGCTCTTCTCAGGGATGG CGTTTGTTGCAGCCATGATGATCCTGGTTCGACCGGTGATGAACCGTGTTGCTCGCAAGTGTTCTCACGA ACAAGACGTGTTACCCGAAATCTACATATGTTTAACTCTAGCGGGAGTAATGTTATCGGGGTTAGTGACA GACATGATAGGGTTACATTCAATTTTCGGGGGATTTGTTTTCGGGCTAACGATACCGAAAGGTGGCGAAT TTGCAAATAGAATGACGAGGAGGATTGAGGACTTCGTGTCCACGTTGTTCCTTCCCTTGTACTTTGCTGC CAGTGGTTTGAAAACTGACGTGACTAAGTTACGAAGCGTGGTGGATTGGGGGCTTCTTTTGCTGGTTACG TCCACCGCGAGCGTGGGGAAGATTTTGGGAACGTTTGCGGTGGCGATGATGTGCATGGTGCCGGTGAGAG AATCCTTGACGCTTGGAGTGTTAATGAACACCAAAGGGTTGGTGGAGCTAATCGTTCTCAATATTGGCAG AGAGAAGAAGGTGCTTAACGACGAGATGTTTACCATCCTAGTACTCATGGCTCTCTTCACCACCTTCATT ACAACTCCAATAGTCTTGGCCATATACAAACCCTCTCGTATAGTAAACTCCGGTTCGCAAAAACCGTCGC GGCTAACAGATTTGCAAGAGAAGCTTCGCATTCTTGCGTGCATCCATGGACCTGGCAACATACCCTCACT AATCAACTTCGTTGAATCAATTCGGGCCACCAACATGTCACGACTCAAACTCTACGTGATGCAACTTACC GAACTCACTGATAGCTCTTCCTCCATCTTGATGGTTCAACGCAGTCGAAAGAATGGTTTTCCCTTCATCA ACCGAATGAAGAGTGGACCAATGCATGAGCAAATTGCCACAGCATTCCAGGCTTATGGTGAAGTGGGTAA AGTTACTGTGCATCATTTAACATCTATCTCTCTATTGTCAACAATGCACGAGGACATATGCCACGTTGCA GAAAAGAAAGGTGTGGCAATGATTATATTGCCCTTCCACAAAAGGTGGGGAGGGGAAGATGAAGAGGTGA CAGAAGACTTAGGGCAGGGTTTGAGGGAAGTCAATCAAAGGGTGCTTCAAAATGCAGCCTGCTCTGTCGC AGTGCTAGTCAATCGTGGGGTTGCCAGAAGGTACGAACAAGAACCTGAGACAAGTGTTGCTGCAAGGAAA AGAGTGTGCATAATTTTCATTGGTGGACCACATGATCGCAAGGTTTTGGAGTTAGGTAGCAGAATGGCAG AGCATCCAGCAATTAGGTTGCTTTTAGTGAGATTCACTTCATACACAGAAGTTGGGGACGAGGGACCCAA ATATAACTCACCAACATCAACCACCAACTGGGAAAAAGAAAAGGAGTTGGATGAGGAAGCAGTAAACGAG TTCAAGGTTAAATGGCAGGAGACTGTGGAGTACATTGAAAAGAACGCAACCAACATAACAGAGGAGGTGT TATCAATTGGGAAAGCTAAGGATCACGACCTAGTAATTGTGGGGAAGCAACAACTTGAGACAACCATGTT GACAAACATAGATTTTCGTCACGGGAATGAAGAGCTGGGACCCATTGGAGATCTCTTTGTCTCTTCGGGT AACGGCATTACCAGTTCATTGCTCGTTATACAGGACCGATATTTTATAAATTCAAACGAAAGTAATCTCG TTAAGACATCAAGGGCCGAGAGTACTGTGATTAAAGATGCTATCGAAGAACTTTAA
  - Protein product >AIG92838.1 CHX1 [Glycine soja] MTFNASTITTASEGAWQGDNPLNHALPLLIVQTILVVFVSRTLAFLLKPFRQPKVVAEIIGGILLGPSAI GRNKKFMHIVFPAWSTTMLESVASFGLLFYLFLVGLELDFRTIRRSGKQAFNIAVAGITLPFICAVGVTF LLQRAIRSENHNIGYVQHFVFLGVSLSITAFPVLARILAELKLLTTRVGETAMAAAAFNDVAAWVLLALA VALAGQGHKSSLLTSIWVLFSGMAFVAAMMILVRPVMNRVARKCSHEQDVLPEIYICLTLAGVMLSGLVT DMIGLHSIFGGFVFGLTIPKGGEFANRMTRRIEDFVSTLFLPLYFAASGLKTDVTKLRSVVDWGLLLLVT STASVGKILGTFAVAMMCMVPVRESLTLGVLMNTKGLVELIVLNIGREKKVLNDEMFTILVLMALFTTFI TTPIVLAIYKPSRIVNSGSQKPSRLTDLQEKLRILACIHGPGNIPSLINFVESIRATNMSRLKLYVMQLT ELTDSSSSILMVQRSRKNGFPFINRMKSGPMHEQIATAFQAYGEVGKVTVHHLTSISLLSTMHEDICHVA EKKGVAMIILPFHKRWGGEDEEVTEDLGQGLREVNQRVLQNAACSVAVLVNRGVARRYEQEPETSVAARK RVCIIFIGGPHDRKVLELGSRMAEHPAIRLLLVRFTSYTEVGDEGPKYNSPTSTTNWEKEKELDEEAVNE FKVKWQETVEYIEKNATNITEEVLSIGKAKDHDLVIVGKQQLETTMLTNIDFRHGNEELGPIGDLFVSSG NGITSSLLVIQDRYFINSNESNLVKTSRAESTVIKDAIEEL
curators:
  - Greg Murrell, Steven Cannon
phenotype_summary: Raises salt tolerance.
traits:
  - entity_name: salt tolerance
    entity: TO:0006001
  - relation_name: positively regulates
    relation: RO:0002213
references:
  - citation: Qi, Li et al., 2014
    doi: 10.1038/ncomms5340
    pmid: 25004933