Run of program pandagma pan, version 2026-06-29 Run started at: Tue Jun 30 09:16:03 AM CDT 2026 Run ended at: Tue Jun 30 01:23:05 PM CDT 2026 Sequence type: nucleotide Parameter value clust_iden 0.90 clust_cov 0.50 extra_iden 0.80 mcl_inflation 1.6 strict_synt 1 pctl_low 25 pctl_med 50 pctl_hi 75 consen_prefix Pisum.pan1 annot_str_regex ([^.]+\.[^.]+\.[^.]+\.[^.]+)\..+ order_method reference preferred_annot ZW6.gnm1.ann1 Output directory for this run: out_pandagma Statistic value == Initial clusters (containing only genes within synteny blocks) Cluster file 06_syn_pan.clust.tsv num_of_clusters 25966 largest_cluster 7 modal_clst_size 3 num_at_mode 18676 seqs_clustered 71678 == Augmented clusters (unanchored sequences added to the initial clusters) Cluster file 12_syn_pan_aug.clust.tsv num_of_clusters 25966 largest_cluster 363 modal_clst_size 3 num_at_mode 18522 seqs_clustered 86990 == Augmented-extra clusters (with sequences from extra annotation sets) The pctl25 set consists of orthogroups with at least 2 genes per OG (>= 4 * 25/100 sets). Cluster file 18_syn_pan_aug_extra.clust.tsv num_of_clusters 25966 largest_cluster 453 modal_clst_size>=2 4 num_at_mode>=2 17467 seqs_clustered 115445 == Sequence stats for CDS files Class: seqs min max N50 ave annotation_name Main: 49597 102 21408 1353 935.8 pissa.Cameor.gnm2.ann1 Main: 33530 6 16200 1509 1156.8 pissa.JI2822.gnm1.ann1 Main: 47133 81 33306 1413 1117.0 pissa.ZW6.gnm1.ann1 Extra: 44674 83 42268 1368 970.2 pissa.Cameor.gnm1.ann1 Avg: 43733 68 28295 1410 1044 all_annot_sets == Sequence stats for final pangene CDS files -- pctl25 and trimmed Class: seqs min max N50 ave annotation_name pctl25: 23941 150 16200 1665 1375.4 23_syn_pan_pctl25_posn_cds.fna == Proportion of initial genes retained in the "aug_extra" and "pctl25" sets: Start End_all End_core Pct_kept_all Pct_kept_core Annotation_name 44674 26024 26024 58.3 58.3 pissa.Cameor.gnm1.ann1 49597 31593 31593 63.7 63.7 pissa.Cameor.gnm2.ann1 33530 29375 29375 87.6 87.6 pissa.JI2822.gnm1.ann1 47133 28453 28453 60.4 60.4 pissa.ZW6.gnm1.ann1 == For all annotation sets, counts of genes-in-orthogroups and counts of orthogroups-with-genes: gns-in-OGs OGs-w-gns OGs-w-gns/gns pct-non-null-OGs pct-null-OGs annot-set 26024 23002 88.39 88.59 11.41 pissa.Cameor.gnm1.ann1 31593 25306 80.10 97.46 2.54 pissa.Cameor.gnm2.ann1 29375 23359 79.52 89.96 10.04 pissa.JI2822.gnm1.ann1 28453 23540 82.73 90.66 9.34 pissa.ZW6.gnm1.ann1 Counts of initial clusters by cluster size, file 06_syn_pan.clust.tsv: 2 6804 3 18676 4 399 5 77 6 9 7 1 Counts of augmented clusters by cluster size, file 12_syn_pan_aug.clust.tsv: 2 4535 3 18522 4 948 5 552 6 571 7 190 8 137 9 126 10 80 11 47 12 29 13 27 14 30 15 21 16 13 17 17 18 9 19 8 20 9 21 10 22 7 23 8 24 2 25 1 26 3 27 1 28 3 29 3 30 1 31 2 33 1 34 2 36 2 37 2 38 1 39 4 40 2 42 1 43 1 44 2 49 1 50 1 51 1 52 1 54 1 55 1 57 1 60 1 62 1 65 1 67 2 69 1 72 1 73 1 77 1 87 1 92 2 98 1 100 1 102 1 103 1 104 1 105 1 108 2 109 1 111 2 116 1 117 1 121 1 130 1 134 1 363 1 Counts of augmented-extra clusters by cluster size, file 18_syn_pan_aug_extra.clust.tsv: 2 1364 3 3977 4 17467 5 804 6 529 7 439 8 456 9 187 10 120 11 118 12 109 13 55 14 30 15 41 16 37 17 25 18 16 19 22 20 10 21 17 22 13 23 9 24 5 25 5 26 10 27 7 28 7 29 5 30 2 31 2 32 1 33 3 34 4 35 1 36 2 37 1 38 1 39 2 40 1 41 1 42 5 43 1 44 6 45 3 47 1 48 2 50 1 51 1 52 1 55 2 57 1 58 1 59 1 62 1 65 1 66 1 67 1 72 2 73 1 77 2 84 1 92 1 98 2 99 1 100 2 102 2 108 1 109 3 111 4 117 1 118 1 120 1 123 1 127 1 149 1 154 1 248 1 453 1