--- identifier: G19833.gnm1.mrk.Hanai_deCampos_2007 synopsis: "Three cDNA libraries generated by the Bean EST project were analyzed for the presence of SSRs. A total of 219 expressed sequence tags (ESTs) were found to carry 240 SSRs (named EST-SSR), whereas 714 genomic sequences contained 471 SSRs (named genomic-SSR)." scientific_name: Phaseolus vulgaris taxid: 3885 scientific_name_abbrev: phavu genotype: - G19833 description: "3 cDNA libraries generated by the Bean EST project (http://lgm.esalq.usp.br/BEST/), comprising a unigene collection of 3126 sequences and a genomic microsatellite-enriched library, were analyzed for the presence of SSRs. A total of 219 expressed sequence tags (ESTs) were found to carry 240 SSRs (named EST-SSR), whereas 714 genomic sequences contained 471 SSRs (named genomic-SSR). A subset of 80 SSRs, 40 EST-SSRs, and 40 genomic-SSRs were evaluated for molecular polymorphism in 23 genotypes of cultivated beans from the Mesoamerican and Andean genetic pools, including Brazilian cultivars and 2 related species. Of the common bean genotypes, 31 EST-SSR loci were polymorphic, yielding 2–12 alleles as compared with 26 polymorphic genomic-SSRs, accounting for 2–7 alleles. Cluster analysis from data using both genic and genomic-SSR revealed a clear separation between Andean and Mesoamerican beans. The usefulness of these loci for distinguishing bean genotypes and genetic mapping is discussed." publication_doi: 10.1139/G07-007 publication_title: "Development, characterization, and comparative analysis of polymorphism at common bean SSR loci isolated from genic and genomic sources" contributors: "Luiz Ricardo Hanai, Tatiana de Campos, Luis Eduardo Aranha Camargo, Luciana Lasry Benchimol, Anete Pereira de Souza, Maeli Melotto, Sergio Augusto Moraes Carbonell, Alisson Fernando Chioratto, Luciano Consoli, Eduardo Fernando Formighieri, Marcos Vinıcius Bohrer Monteiro Siqueira, Siu Mui Tsai, and Maria Lucia Carneiro Vieira" citation: "Hanai, L. R. et al. Development, characterization, and comparative analysis of polymorphism at common bean SSR loci isolated from genic and genomic sources. Genome 50, 266–277 (2007)" data_curators: Sam Hokin public_access_level: public license: Open genotyping_platform: Hanai_deCampos_2007