Run of program pandagma-pan.sh, version 2023-08-28 Run started at: Sat Sep 2 17:23:50 CDT 2023 Run ended at: Sat Sep 2 17:47:12 CDT 2023 Sequence type: nucleotide Parameter value clust_iden 0.90 clust_cov 0.50 consen_iden 0.80 extra_iden 0.80 mcl_inflation 1.6 strict_synt 1 dagchainer_args -g 10000 -M 50 -D 200000 -E 1e-5 -A 6 -s out_dir_base out pctl_low 25 pctl_med 50 pctl_hi 75 consen_prefix Phaseolus.pan2 annot_str_regex ([^.]+\.[^.]+\.[^.]+\.[^.]+)\..+ order_method alignment preferred_annot G19833.gnm2.ann1 work_dir /project/legume_project/steven.cannon/pandagma/Phaseolus/../work_Phaseolus Output directory for this run: out_Phaseolus_7_1 Statistic value == Initial clusters (containing only genes within synteny blocks) Cluster file 06_syn_pan.clust.tsv num_of_clusters 26790 largest_cluster 22 modal_clst_size 7 num_at_mode 16936 seqs_clustered 164464 == Augmented clusters (unanchored sequences added to the initial clusters) Cluster file 12_syn_pan_aug.clust.tsv num_of_clusters 28690 largest_cluster 78 modal_clst_size 7 num_at_mode 17819 seqs_clustered 177265 == Augmented-extra clusters (with sequences from extra annotation sets) The pctl25 set consists of orthogroups with at least 3 genes per OG (>= 8 * 25/100 sets). Cluster file 18_syn_pan_aug_extra.clust.tsv num_of_clusters 28690 largest_cluster 87 modal_clst_size>=3 8 num_at_mode>=3 17621 seqs_clustered 203268 == Sequence stats for CDS files Class: seqs min max N50 ave annotation_name Main: 27538 171 15285 1584 1251.6 phaac.Frijol_Bayo.gnm1.ann1 Main: 27095 6 15285 1608 1231.1 phaac.W6_15578.gnm2.ann1 Main: 33734 90 16293 1620 1250.1 phalu.G27455.gnm1.ann1 Main: 27065 72 16701 1626 1302.9 phavu.5-593.gnm1.ann1 Main: 27433 90 16290 1602 1258.8 phavu.G19833.gnm2.ann1 Main: 27218 90 16359 1623 1295.4 phavu.LaborOvalle.gnm1.ann1 Main: 27385 90 16290 1626 1295.0 phavu.UI111.gnm1.ann1 Extra: 27197 96 16290 1614 1273.7 phavu.G19833.gnm1.ann1 Avg: 28083 88 16099 1612 1269 all_annot_sets == Sequence stats for final pangene CDS files -- pctl25 and trimmed Class: seqs min max N50 ave annotation_name pctl25: 27580 90 18573 1665 1325.3 23_syn_pan_pctl25_posn_cds.fna == Proportion of initial genes retained in the "aug_extra" and "pctl25" sets: Start End_all End_core Pct_kept_all Pct_kept_core Annotation_name 27538 24925 24744 90.5 89.9 phaac.Frijol_Bayo.gnm1.ann1 27095 23984 23836 88.5 88.0 phaac.W6_15578.gnm2.ann1 33734 23452 22761 69.5 67.5 phalu.G27455.gnm1.ann1 27065 26588 26554 98.2 98.1 phavu.5-593.gnm1.ann1 27197 26003 26003 95.6 95.6 phavu.G19833.gnm1.ann1 27433 25555 25548 93.2 93.1 phavu.G19833.gnm2.ann1 27218 26467 26344 97.2 96.8 phavu.LaborOvalle.gnm1.ann1 27385 26294 26234 96.0 95.8 phavu.UI111.gnm1.ann1 == For all annotation sets, counts of genes-in-orthogroups and counts of orthogroups-with-genes: gns-in-OGs OGs-w-gns OGs-w-gns/gns pct-non-null-OGs pct-null-OGs annot-set 24925 23329 93.60 81.31 18.69 phaac.Frijol_Bayo.gnm1.ann1 23984 22644 94.41 78.93 21.07 phaac.W6_15578.gnm2.ann1 23452 22297 95.08 77.72 22.28 phalu.G27455.gnm1.ann1 26588 24681 92.83 86.03 13.97 phavu.5-593.gnm1.ann1 26003 24797 95.36 86.43 13.57 phavu.G19833.gnm1.ann1 25555 24245 94.87 84.51 15.49 phavu.G19833.gnm2.ann1 26467 24855 93.91 86.63 13.37 phavu.LaborOvalle.gnm1.ann1 26294 24993 95.05 87.11 12.89 phavu.UI111.gnm1.ann1 Counts of initial clusters by cluster size, file 06_syn_pan.clust.tsv: 2 2217 3 1147 4 960 5 1298 6 3571 7 16936 8 279 9 119 10 99 11 79 12 42 13 21 14 8 15 5 16 4 17 3 18 1 22 1 Counts of augmented clusters by cluster size, file 12_syn_pan_aug.clust.tsv: 2 2390 3 1631 4 1277 5 1243 6 2919 7 17819 8 681 9 208 10 119 11 123 12 94 13 70 14 37 15 20 16 12 17 12 18 4 19 6 20 3 21 5 22 4 24 2 25 2 28 2 32 1 36 1 37 2 44 1 50 1 78 1 Counts of augmented-extra clusters by cluster size, file 18_syn_pan_aug_extra.clust.tsv: 2 1783 3 1527 4 1087 5 1031 6 1204 7 2903 8 17621 9 729 10 226 11 136 12 100 13 88 14 84 15 62 16 28 17 17 18 10 19 11 20 10 21 5 22 3 23 3 24 4 25 1 26 2 27 2 28 1 29 1 30 1 33 3 37 1 42 1 43 1 45 1 50 1 55 1 87 1 # Number of annotations per pangene bin abcdefghijKLMNOPQRSTabcdefghijKLMNOPQRSTabcdefghijKLMNOPQRSTabcdefghijKLMNOPQRSTabcdefghijKLMNOPQRST 1.00 .. 2.00 .......... 3.00 ........ 4.00 .... 5.00 ..... 6.00 ...... 7.00 .................. 8.00 ......................................................................................