---
identifier: Tifrunner.gnm1.met1.5B59

provenance: "The files in this directory are considered the primary instances, but they are also initial assembly versions, prior to deposition into GenBank - which will then become the primary, authoritative instances. The files here are held as part of the PeanutBase, LegumeInfo, and LegumeFederation projects. Please also see the associated usage_policy.txt Briefly: By accessing these data, you agree not to publish any articles containing analyses of genes or genomic data on a whole genome or chromosome scale prior to publication by the PGC and/or its collaborators of a comprehensive genome analysis (Reserved Analyses). See what is considered Reserved Analyses in the usage policy."

source: "https://peanutbase.org/data/"

synopsis: Methylation states of the bases in the Tifrunner genome.

scientific_name: Arachis hypogaea

taxid: 3818

bioproject: PRJNA291488

scientific_name_abbrev: arahy

genotype:
  - Tifrunner

description: "MethylC-seq libraries were constructed as described in the publication. Size selection was performed using magnetic purification beads. The End-It DNA End-Repair Kit (Epicentre) was used to perform end repair on the fragmented DNA. A-tails were added to blunt-end fragments using Klenow 3'-5' exonuclease and dA-Tailing Buffer (New England Biolabs). Methylated NEXTflex DNA adapters (Bio Scientific) were then ligated on using T4 DNA ligase (New England Biolabs). Bisulfite conversion was done using the MethylCode Bisulfite Conversion Kit (Invitrogen). Between each reaction, magnetic purification beads were used to clean up the DNA. MethylC-seq libraries were sequenced on an Illumina HiSeq 2500. See the publication (Supplementary Methods) for additional details."

genbank_accession: PIVG00000000

original_file_creation_date: "2018-06-25"

local_file_creation_date: "2018-06-25"

dataset_release_date: "2018-08-16"

publication_doi: 10.1007/978-3-319-63935-2_8

contributors: "The International Peanut Genome Initiative; lead assembly group Jeremy Schmutz, Jerry Jenkins, Jane Grimwood; project leads David Bertioli; Soraya Bertioli; Brian Schleffler; Scott Jackson; Peggy Ozias-Akins"

data_curators: Ethalinda Cannon, Steven Cannon, Jerry Jenkins, JD Campbell

public_access_level: public

license: Open, with usage agreement

keywords: peanut, Arachis hypogaea, Tifrunner, methylation